Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
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چکیده
منابع مشابه
AtWuschel Promotes Formation of the Embryogenic Callus in Gossypium hirsutum
Upland cotton (Gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. Callus from only a few cultivars can produce embryogenic callus (EC), but the mechanism is not well elucidated. Here we screened a cultivar, CRI24, with high efficiency of EC produce. The expression of genes relevant to EC production was analyzed between the ...
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The objective of this study was to increase understanding about the mechanism by which polyamines (PAs) promote the conversion of embryogenic calli (EC) into somatic embryos in cotton (Gossypium hirsutum L.). We measured the levels of endogenous PAs and H2O2, quantified the expression levels of genes involved in the PAs pathway at various stages of cotton somatic embryogenesis (SE), and investi...
متن کاملBiochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.
Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...
متن کاملLabel-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis
The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...
متن کاملQTL analysis for early-maturing traits in cotton using two upland cotton (Gossypium hirsutum L.) crosses
Making use of the markers linked closely to QTL for early-maturing traits for MAS (Marker-assisted selection) is an effective method for the simultaneous improvement of early maturity and other properties in cotton. In this study, two F2 populations and their F2:3 families were generated from the two upland cotton (Gossypium hirsutum L.) crosses, Baimian2 × TM-1 and Baimian2 × CIR12. QTL for ea...
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ژورنال
عنوان ژورنال: BMC Developmental Biology
سال: 2020
ISSN: 1471-213X
DOI: 10.1186/s12861-020-00230-4